Highly sensitive detection of hydrazine by a disposable, Poly(Tannic Acid)-Coated carbon electrode

Electrochemical sensor based on the enhanced electrocatalytic oxidation exhibited on a functionalized poly (tannic acid) coating to detect hydrazine.


Hydrazine is very toxic, colorless, flammable, easily water-soluble, and can cause severe injuries to lungs, liver, kidneys, brain, and spinal cord in humans. It has been classified as a probable human carcinogen in group B2 by the United States Environmental Protection Agency, which has set its threshold limit value as low as 10 ppb (312 nM).

Therefore, the detection and monitoring of trace amounts of HZ discharged in the environment is very important for environmental and biological analysis. Numerous methods have been reported on the sensitive and selective trace-level detection of HZ. Electrochemical techniques are superior in terms of simplicity, sensitivity, speed, low cost, and being compatible with miniaturized portable devices for on-site analysis. However, direct electrochemical oxidation of HZ on bare conventional electrode surfaces leads to sluggish kinetics and high over-potentials.

Electrocatalytic detection of hydrazine of poly (tannic acid)-coated, screen-printed carbon electrode surface


Prof. Cho and a team has presented a simple, rapid, and cheap electrode functionalization method for HZ detection based on an enhanced electrocatalytic oxidation of HZ on poly (tannic acid)-functionalized electrodes. The poly (tannic acid) was prepared by a rapid (30 min) enzymatic polymerization with horseradish peroxidase (HRP) and drop-casted on a disposable screen-printed carbon electrode (SPCE) with a short incubation period


The study has been supported through the IBS Center for Soft and Living Matter. This work is detailed in a paper published in the Biosensors and Bioelectronics (Al-MonsurJiaul Haque et al., Highly sensitive detection of hydrazine by a disposable, Poly (Tannic Acid)-Coated carbon electrode, Biosensors and Bioelectronics), DOI: 10.1016/j.bios.2019.111927.