The SWI/SNF Chromatin RemoThe SWI/SNF Chromatin Remodeling Complex, Lymphocyte Differentiation, and Tumordeling Complex, Lymphocyte Differentiation, and Tumor
Seong, Rho Hyun Affiliation : Seoul National University Date : 26.05.2016 4:00 PM Place : Engineering Building E104 Contact : firstname.lastname@example.org
The Swi/Snf chromatin remodeling complex is a group of epigenetic regulators that physically disrupt DNA-histone contacts to regulate gene expression by using energy derived from ATP hydrolysis. The Swi/Snf complex is composed of multiple subunits including Brg1 with ATPase activity, Srg3/mBaf155, Baf170, and Baf47/Snf5 as the core subunits of the complex. SRG3, a murine homolog of human BAF155, serves as a scaffold protein that controls the stability of the SWI/SNF complex through direct interaction with the other subunits of the complex.
We found that the SWI/SNF chromatin remodeling complex is required for GC reactions. The genetic ablation of Srg3/mBaf155, a core component of the SWI/SNF complex, impairs GC B and follicular helper T (TFH) cell development. The SWI/SNF complex cooperatively interacts with Bcl-6 and mediates Blimp-1 repression through facilitating the chromatin conformation and histone modification. I will also present our work showing that the Swi/Snf complex is required for Th17 cell differentiation. The Swi/Snf complex acts directly on the IL-17A/IL-17F locus and plays an essential role for modulating chromatin accessibility in this region. We also uncovered a role of RORγt as a major chromatin regulator of IL-17A/IL-17F locus in Th17 cell differentiation by recruiting the SWI/SNF complex. My presentation will also cover that Brg1 is a functional target of miR-139 and that Brg1 is related to BCR-ABL-induced leukemogenesis.